Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Psychopharmacology Debates: Tylenol or NSAIDs? Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Glutathione, Tylenol & Your Health: What You Should Know TylenolPartner 97 shows in under 5 months yeah my body feels it but we keep going thanks to @tylenol for aches and pains Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products